Western blot analysis of GABBR1 in different various lysates. Lane 1: K-562 cell lysate; Lane 2: Jurkat cell lysate; Lane 3: HeLa cell lysate; Lane 4: SHG-44 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 108 kDa. Observed band size: 108 kDa. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Primary antibody GABBR1 recombinant monoclonal antibody (Product # MA5-50825) with a dilution of 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:200,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.